ABSTRACT
Hydroalcoholic extract [70% ethanol extract] of tubers of Momordica tuberosa Cogn. [Cucurbitaceae] was subjected to preliminary phytochemical screening by qualitative tests. Nitric oxide scavenging activity was performed by Griess reagent method. And nephroprotective activity was assessed in gentamicin, cisplatin and paracetamol induced renal damage in wistar rats [150-200 g] by standard methods. The protective property of 70% ethanol extract was assessed by measuring the levels of body weight, blood urea, serum creatinine, tissue glutathione and lipid peroxidation in administered doses. The extract exhibited free radical scavenging activity in dose dependant manner. And 100 micro g/ml dose produced significantly higher scavenging activity than standard sodium metabisulphate at 25 micro g/ml. Also, it significantly reduced the renal damage caused by cisplatin, gentamicin and paracetamol at a dose of 40 mg/kg
Subject(s)
Animals, Laboratory , Male , Female , Phytotherapy , Plant Extracts , Nitric Oxide , Cucurbitaceae , Renal Insufficiency , Cisplatin/toxicity , Free Radical Scavengers , Blood Urea Nitrogen , Gentamicins/toxicity , Acetaminophen/toxicity , Rats , Lipid PeroxidationABSTRACT
The study was aimed at establishing pharmacognostical profile for the tubers of plant Momordica tuberosa Cogn., Cucurbitaceae. Morphoanatomy of tubers of this plant were studied in order to establish its complete profile to aid in its identification and avoid confusion in its taxanomic species. These were established using light microscopy, WHO recommended physicochemical and phytochemical procedures. The parameters presented here may be used to establish the authenticity of tubers of this plant as this part has been used traditionally in India and also to differentiate between closely related Momordica species.
Este trababalho teve como objetivo estabelecer um perfil farmacognóstico dos tuvérculos da planta Momordica tuberosa Cogn., Cucurbitaceae. A morfoanatomia dos tubérculos da espécie foi realizada para estabelecer um perfil completo que possa ajudar na identificação e evitar problemas na sua taxonomia. Os ensaios foram realizados usando microscópio ótico, procedimentos fisico-químicos e fitoquímicos estabelecidos pela OMS. Os parâmetros apresentados podem ser usados para apresetar a autenticidade dos tubérculos desta espécie, uma vez que esta parte da droga é utilizada tradicionalmente na Índia e, também, para diferenciá-la de outras espécies de Momordica.